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KMID : 0903619970380020129
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Journal of the Korean Society for Horticultural Science
1997 Volume.38 No. 2 p.129 ~ p.132
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Intraspecific Variations fo the Allium victorialis var . platyphyllum by Polymerase Chain Reaction
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김원배/Kim, Won Bae
임학태/유기억/류승열/서종택/엄영현/Yoo, Ki Oug/Ryu, Seung Yeol/Seo, Jong Taek
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Abstract
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The intraspecific variations among Allium victorialis var. platyphyllum populations including habitats and 41 individuals were investigated based on the randomly amplified DNA polymorphisms derived from PCR (polymerase chain reaction) method. Eleven primers out of 81 primers (80 random [10-mer] primers, one 15-mer[M13 core sequence] primer) screened resulted in DNA polymorphisms (61.5/0) in all of the tested plants, producing R3 randomly and specifically amplified DNA fragments. Ulleungdo population was discrete from the other two populations at the 0.77 of similarity index value of DNA polymorphisms. But, Jirisan and Odaesan populations were not differentiated from each other. For the variation of each population, Ulleungdo population showed lower level of variation with similarity index of 0.92 than Jirisan and Odaesan populations with 0.86 and 0.87 respectively.
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KEYWORD
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